CST, 4672S, JNK2 Antibody

Polyclonal Antibody for studying JNK2. Validated for Western Blotting. Highly specific and rigorously validated in-house, JNK2 Antibody (CST #4672) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity JNK2 Antibody detects endogenous levels of JNK2. This antibody will only recognize the JNK2 Alpha 2 and JNK2 Beta 2 isoforms. This antibody will not recognize JNK2 Alpha 1 and JNK2 Beta 1 isoforms. This antibody does not recognize p38 MAP kinase, p44/42MAP kinase or JNK1. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human JNK2. Antibodies are purified by protein A and peptide affinity chromatography. Background The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). Alternate Names c-Jun kinase 2; c-Jun N-terminal kinase 2; JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; Jun kinase; MAP kinase 9; MAPK 9; MAPK9; Mitogen-activated protein kinase 9; mitogen-activated protein kinase 9 isoform JNK2 alpha2; MK09; p54a; p54aSAPK; PRKM9; SAPK; SAPK1A; Stress-activated protein kinase 1a; Stress-activated protein kinase JNK2 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 54 SOURCE: Rabbit