CST, 46743S, NRF1 (D9K6P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NRF 1. Validated for WB,WB,IP,ChIP,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, NRF1 (D9K6P) Rabbit Monoclonal Antibody (CST #46743) is ready to ship. Product Usage Information For optimal ChIP and ChIP-Seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Chromatin IP-seq: 1:100 CUT&RUN: 1:50 CUT&Tag: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq, CUT&RUN, CUT&Tag Specificity / Sensitivity NRF1 (D9K6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total NRF1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala175 of human NRF1 protein. Background Nuclear respiratory factor 1 (NRF1) was identified as a transcription activator for the gene encoding cytochrome c (1). It was later found to play a role in the nuclear control of mitochondrial function (1). PGC-1 induces the expression of NRF1 and NRF2 (2). NRF1, along with the coactivator PGC-1, stimulates the promoter of mitochondrial transcription factor A, which regulates mitochondrial biogenensis and function (2). Alternate Names Alpha palindromic-binding protein; Alpha-pal; NRF-1; NRF1; Nuclear respiratory factor 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG