CST, 4680S, BMP4 (6B7) Mouse Monoclonal Antibody

Monoclonal Antibody for studying BMP4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, BMP4 (6B7) Mouse Monoclonal Antibody (CST #4680) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BMP4 (6B7) Mouse Monoclonal Antibody detects transfected levels of total BMP4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a BMP4 fusion protein. Background Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belong to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the C-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5, and 8), resulting in the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4). BMP4 plays an essential role during development. Mouse embyros deficient in BMP4 die around the time of gastrulation (5). BMP4 regulates stem cell maintenance and differentiation in various systems (6-8). Alternate Names BMP-2B; BMP-4; BMP2B; BMP2B1; BMP4; Bone morphogenetic protein 2B; Bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 47 Source/Isotype: Mouse IgG1