CST, 4681S, TLE1/2/3/4 Antibody

Polyclonal Antibody for studying TLE3/TLE4/TLE2/TLE1. Validated for Western Blotting. Highly specific and rigorously validated in-house, TLE1/2/3/4 Antibody (CST #4681) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TLE1/2/3/4 Antibody detects endogenous levels of TLE1, TLE2, TLE3, and TLE4 proteins. Based on homology this antibody should also recognize TLE1 and TLE2 of Zebra fish, TLE2 of Xenopus, and Groucho of Drosophila. Species Reactivity: Human, Mouse, Monkey, D. melanogaster Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp760 of human TLE1. Antibodies are purified by peptide affinity chromatography. Background Transducin-like enhancer of split proteins (TLE1, TLE2, TLE3, TLE4, and TLE6) are mammalian homologs of Drosophila Groucho (1). TLEs contain several WD-repeats implicated in protein-protein interaction (2,3). TLEs are transcriptional co-repressors that bind to many transcription factors, such as LEF1, Runx1, Oct-1, hepatocyte nuclear factor 3-β as well as histone H3 (4-7). TLEs are differentially expressed during animal development and may have overlapping as well as distinct functions (8). Alternate Names B lymphocyte gene 1; BCE-1; BCE1; E(Sp1) homolog; E(spI); E(spl); enhancer of split groucho 1; enhancer of split groucho 2; enhancer of split groucho 3; enhancer of split groucho 4; Enhancer of split groucho-like protein 1; Enhancer of split groucho-like protein 2; Enhancer of split groucho-like protein 3; ESG; ESG1; ESG2; ESG3; ESG4; FLJ39460; FLJ41188; Grg-4; GRG1; GRG2; GRG3; GRG4; Groucho-related protein 4; HsT18976; KIAA1261; KIAA1547; Protein BCE-1; TLE family member 1, transcriptional corepressor; TLE family member 2, transcriptional corepressor; TLE family member 3, transcriptional corepressor; TLE family member 4, transcriptional corepressor; TLE1; TLE2; TLE3; TLE4; transducin like enhancer of split 1; transducin like enhancer of split 2; transducin like enhancer of split 3; transducin like enhancer of split 4; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 1, homolog of Drosophila E(sp1); transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 2, homolog of Drosophila E(sp1); transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 3, homolog of Drosophila E(sp1); transducin-like enhancer of split 4; transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 4, homolog of Drosophila E(sp1); Transducin-like enhancer protein 1; Transducin-like enhancer protein 2; Transducin-like enhancer protein 3; Transducin-like enhancer protein 4 Specification REACTIVITY: H M Mk Dm SENSITIVITY: Endogenous MW (kDa): 78-90 SOURCE: Rabbit