CST, 46843S, LAMP1 (E6N3R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAMP1 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP1 (E6N3R) Rabbit Monoclonal Antibody (CST #46843) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity LAMP1 (E6N3R) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMP1 protein. Non-specific nucleolar staining was observed in mouse brain by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe122 of mouse LAMP1 protein. Background Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). Alternate Names 120 kDa lysosomal membrane glycoprotein; AI196048; CD107; CD107 antigen-like family member A; CD107a; Lam; Lamp I; LAMP-1; Lamp1; LGP-120; LGP-A; lysosomal membrane glycoprotein 1; Lysosomal membrane glycoprotein A; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); Lysosome-associated membrane protein 1; P2B; Perk Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 42 (non-glycosylated), 90-120 (glycosylated) Source/Isotype: Rabbit IgG