CST, 46862S, MYST1 (D5T3R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYST1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MYST1 (D5T3R) Rabbit Monoclonal Antibody (CST #46862) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MYST1 (D5T3R) Rabbit Monoclonal Antibody recognizes endogenous levels of total MYST1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Val80 of human MYST1 protein. Background MYST1, also known as mammalian male absent on the first (MOF) and lysine acetyltransferase 8 (KAT8), is a member of the MYST (MOZ, YBF2, SAS2 and Tip60) family of histone acetyltransferases (1,2). As the catalytic subunit of two different histone acetyltransferase complexes, MSL and NSL, MYST1 is responsible for the majority of histone H4 lysine 16 acetylation in the cell. MYST1 also acetylates p53 on lysine 120 and is important for activation of pro-apoptotic genes (1,2). As a component of the MSL complex, MYST1 associates with MSL1, MSL2L1, and MSL3L1, and specifically acetylates histone H4 on lysine 16 (3-5). As part of the NSL complex, MYST1 associates with the MLL1 histone methyltransferase complex containing MLL1/KMT2A, ASH2L, HCFC1, WDR5 and RBBP5, and shows broader acetyltransferase activity for histone H4 on lysines 5, 8, and 16 (3-5). MYST1 plays a critical role in the regulation of transcription, DNA repair, autophagy, apoptosis, and emybryonic stem cell pluripotency and differentiation (1,2,6). Loss of MYST1 leads to a global reduction in histone H4 lysine 16 acetylation, a common hallmark found in many human cancers. A reduction of MYST1 protein levels and histone H4 lysine 16 acetylation is associated with poor prognosis in breast, renal, colorectal, gastric, and ovarian cancers (1). Alternate Names FLJ14040; Histone acetyltransferase KAT8; histone acetyltransferase MYST1; hMOF; K(lysine) acetyltransferase 8; KAT8; LIGOWS; Lysine acetyltransferase 8; MOF; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1; MYST histone acetyltransferase 1; MYST-1; MYST1; ortholog of Drosophila males absent on the first (MOF); Probable histone acetyltransferase MYST1; ZC2HC8 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 53 Source/Isotype: Rabbit IgG