CST, 4688T, cdc25C (5H9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cdc25c. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, cdc25C (5H9) Rabbit Monoclonal Antibody (CST #4688) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity cdc25C (5H9) Rabbit Monoclonal Antibody detects endogenous levels of total cdc25C protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a cdc25C full length fusion protein. Â Background Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). Alternate Names CDC25; CDC25C; cell division cycle 25 homolog C (S. pombe); cell division cycle 25C; Dual specificity phosphatase Cdc25C; M-phase inducer phosphatase 3; mitosis inducer CDC25; MPIP3; phosphotyrosine phosphatase; PPP1R60; protein phosphatase 1, regulatory subunit 60 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60, 75 Source/Isotype: Rabbit IgG