CST, 46890S, Granzyme B (D6E9W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Granzyme B. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Granzyme B (D6E9W) Rabbit Monoclonal Antibody (CST #46890) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 79903 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Granzyme B (D6E9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total Granzyme B protein. Non-specific staining in the sweat glands has been observed.This antibody recognizes human Granzyme B protein and is also reactive with mouse Granzyme B; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Instead, Granzyme B (E5V2L) Rabbit Monoclonal Antibody #44153 is recommended for IHC analysis of mouse tissue samples. Monkey cross-reactivity is determined by immunohistochemistry. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human Granzyme B protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Alternate Names C11; Cathepsin G-like 1; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA-1; CTLA1; CTSGL1; cytotoxic serine protease B; Cytotoxic T-lymphocyte proteinase 2; fragmentin 2; Fragmentin-2; GRAB; Granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; GRB; GZMB; HLP; Human lymphocyte protein; Lymphocyte protease; SECT; T-cell serine protease 1-3E Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG