CST, 4689S, DKK2 (N70) Antibody

Polyclonal Antibody for studying Dkk2. Validated for Western Blotting. Highly specific and rigorously validated in-house, DKK2 (N70) Antibody (CST #4689) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DKK2 (N70) Antibody detects transfected levels of human DKK2 protein. This antibody does not cross-react with DKK1. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn70 of human DKK2. Antibodies are purified by peptide affinity chromatography. Background Dickkopf (DKK) family proteins consist of four members (DKK1, DKK2, DKK3, and DKK4) that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6). DKK2 plays a role in osteoblast terminal differentiation and formation of mineralized matrix and is required for differentiation of non-keratinizing stratified epithelium from the corneal epithelial progenitors (7,8). Alternate Names dickkopf 2; dickkopf 2 homolog; dickkopf homolog 2; dickkopf related protein-2; dickkopf WNT signaling pathway inhibitor 2; dickkopf-2; Dickkopf-related protein 2; Dkk-2; DKK2; hDkk-2 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 28, 32 SOURCE: Rabbit