CST, 4690S, SFRP1 Antibody

Polyclonal Antibody for studying SFRP1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SFRP1 Antibody (CST #4690) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SFRP1 Antibody detects endogenous levels of total SFRP1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly48 of human SFRP1. Antibodies are purified by protein A and peptide affinity chromatography. Background Secreted Frizzled-related proteins (SFRPs) display homology and structural similarity to the extracellular cysteine-rich Wnt-binding domain of the G protein-coupled receptor Frizzled (1,2). To date, five distinct SFRPs (SFRP1 to 5) have been found in mammalian cells. These secreted proteins typically act as antagonists to Wnt signaling by directly binding and inhibiting Wnt proteins, or by binding Frizzled to block Wnt protein interaction with the receptor (3). The various SFRPs bind and regulate Wnt proteins differentially; these proteins also display distinct expression patterns as they play important roles in regulating development (4-7). SFRP proteins appear to act as tumor suppressors, with loss of expression or function correlating with many invasive forms of cancer. Deletion of the corresponding SFRP1 gene and promoter hypermethylation leading to gene silencing has been reported in a number of cancers. Abnormal expression of SRFP1 and other Wnt signaling proteins is associated with some cases of retinitis pigmentosa (reviewed in 8). Alternate Names frizzled-related protein; FRP; FRP-1; FRP1; FrzA; SARP-2; SARP2; Secreted apoptosis-related protein 2; secreted frizzled related protein 1; Secreted frizzled-related protein 1; sFRP-1; SFRP1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 SOURCE: Rabbit