CST, 4693S, BMP7 Antibody

Polyclonal Antibody for studying BMP7. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, BMP7 Antibody (CST #4693) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BMP7 Antibody detects transfected levels of total mouse BMP7 protein. This antibody only recognizes the BMP7 precursor protein (49 kDa), not the mature secreted from (16 kDa). Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly99 of mouse BMP7. Antibodies are purified by protein A and peptide affinity chromatography. Background Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belong to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the C-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5, and 8), resulting in the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4). BMP7, also known as osteogenic protein-1 (OP-1), is found to be upregulated in some cancer cells (5-7), and may play a role in cancer metastasis (7-9). Alternate Names BMP-7; BMP7; Bone morphogenetic protein 7; OP-1; OP1; Osteogenic protein 1 Specification REACTIVITY: M SENSITIVITY: Transfected Only MW (kDa): 49 SOURCE: Rabbit