CST, 46993T, TCF4/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (ChIP Formulated)

Monoclonal Antibody for studying ITF2. Validated for Chromatin IP,CUT&RUN,CUT&Tag. Available in 2 sizes. Highly specific and rigorously validated in-house, TCF4/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (ChIP Formulated) (CST #46993) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Chromatin IP: 1:100 CUT&RUN: 1:100 CUT&Tag: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Chromatin IP, CUT&RUN, CUT&Tag Specificity / Sensitivity TCF4/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (ChIP Formulated) recognizes endogenous levels of total TCF4/ITF2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro653 of human TCF4/ITF2 protein. Background Transcription factor 4 (TCF4), also known as immunoglobulin transcription factor 2 (ITF2), is a member of the basic helix-loop-helix family and interacts with Ephrussi-box (E-box) binding sites (1,2). TCF4 is broadly expressed, and due to alternative splicing, transcripts can give rise to over a dozen different isoforms (3). It plays a key role in neuronal development and has been implicated in a variety of developmental disorders, including Pitt-Hopkins syndrome, schizophrenia, and autism (4-9). In addition to its functions in the brain, TCF4 is essential for the development of plasmacytoid dendritic cells (10-12). Finally, in the context of cancer, TCF4 expression may be associated with divergent patient outcomes; higher TCF4 expression is associated with increased survival in non-small cell lung cancer, yet the protein has also recently been reported to confer immunotherapy resistance in melanoma (13,14). Alternate Names BHLHB19; CDG2T; Class B basic helix-loop-helix protein 19; E2-2; FECD3; Immunoglobulin transcription factor 2; ITF-2; ITF2; MGC149723; MGC149724; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; SL3-3 enhancer factor 2; TCF-4; TCF4; Transcription factor 4 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG