CST, 47061T, CELSR2 (D2M9H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CELSR2. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CELSR2 (D2M9H) Rabbit Monoclonal Antibody (CST #47061) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 19983 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CELSR2 (D2M9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CELSR2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His1781 of human CELSR2 protein. Background CELSR2 (cadherin EGF LAG seven-pass G-type receptor, also known as flamingo homolog 3 or epidermal growth factor-like protein 2) is a member of the flamingo subfamily of non-classical cadherins, part of the cadherin superfamily. CELSR2 is a 7-transmembrane helix receptor that contains nine cadherin-like domains, seven EGF-like repeats, and 2 laminin A G-type repeats (1). It shares structural characteristics of both an adhesion molecule and a G protein-coupled receptor, suggesting putatives roles in both cell-cell adhesion and juxtacrine signaling. It's function has been associated with dendrite morphogenesis (2), neural plate anterior-posterior pattern formation (3), and regulation of transcription via the Wnt signaling pathway (4). In a loss-of-function mouse model, Celsr2 deletion resulted in defects in the planar organization of ependymal cilia, leading to defective cerebrospinal fluid dynamics and hydrocephalus (5). In humans, SNPs in the CELSR2 gene cluster on chromosome 1 have been associated with enhanced risk of coronary artery disease (6). Alternate Names ADGRC2; adhesion G protein-coupled receptor C2; Cadherin EGF LAG seven-pass G-type receptor 2; Cadherin family member 10; cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila); CDHF10; CELR2; CELSR2; EGF-like protein 2; EGF-like-domain, multiple 2; EGFL2; epidermal growth factor-like 2; Epidermal growth factor-like protein 2; Flamingo homolog 3; Flamingo1; FLJ34118; FLJ42737; FLJ45143; FLJ45845; KIAA0279; MEGF3; Multiple EGF-like domains protein 3; multiple epidermal growth factor-like domains 3; Multiple epidermal growth factor-like domains protein 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 320 Source/Isotype: Rabbit IgG