CST, 47066T, YKL-40 (E2L1M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying YKL-40. Validated for WB,WB,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, YKL-40 (E2L1M) Rabbit Monoclonal Antibody (CST #47066) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 44073 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity YKL-40 (E2L1M) Rabbit mAb recognizes endogenous levels of total YKL-40 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human YKL-40 protein. Background YKL-40, also known as chitinase-3-like protein 1 (CHI3L1), is a secreted glycoprotein encoded by the gene (1,2). Unlike other members of the chitinase-like protein family, YKL-40 is not an active chitinase. It is produced by activated macrophages, chondrocytes, neutrophils, and synovial cells. It is a pro-inflammatory molecule that contributes to the progression of many inflammatory diseases, including fibrosis, neurodegenerative disease, breast, lung, prostate, liver, bladder, colon, and other types of cancers (3,4). Levels of YKL-40 in serum and plasma in patients with various types of tumors have been reported to predict poor prognosis, and in some clinical assays, YKL-40 is being used as a predictive biomarker of cancer outcome (4). Alternate Names 39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP-39; CH3L1; CHI3L1; chitinase 3 like 1; chitinase 3-like 1; chitinase 3-like 1 (cartilage glycoprotein-39); Chitinase-3-like protein 1; DKFZp686N19119; FLJ38139; GP-39; GP39; HC-gp39; hCGP-39; HCGP-3P; YK-40; YKL-40; YKL40; YYL-40 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-40 Source/Isotype: Rabbit IgG