CST, 47074S, CD162/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SELPLG. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CD162/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody (CST #47074) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Live) Specificity / Sensitivity CD162/PSGL-1 (F8D5X) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD162/PSGL-1 protein. This antibody reacts with monomeric and dimeric forms of CD162/PSGL-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly281 of human CD162/PSGL-1 protein. Background P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is a disulfide-linked homodimer expressed on the cell surface of lymphoid and myeloid cells as a type I transmembrane sialoglycoprotein (1,2). The mature protein backbone of the extracellular domain of PSGL-1 is extensively modified with N-linked glycans, O-linked glycans, sulfated tyrosine residues, and sialyl-Lewis X epitopes (3). Collectively, these modifications modulate high affinity interactions between PSGL-1 and multiple selectins, which facilitates immune cell trafficking to primary and secondary lymphoid organs (4-8). Alternate Names CD162; CLA; cutaneous lymphocyte-associated associated antigen; P-selectin glycoprotein ligand 1; PSGL-1; PSGL1; Selectin P ligand; SELPL; SELPLG Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 240, 120 Source/Isotype: Rabbit IgG