CST, 4707S, Integrin beta4 Antibody

Polyclonal Antibody for studying Integrin beta4. Validated for Western Blotting. Highly specific and rigorously validated in-house, Integrin beta4 Antibody (CST #4707) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Integrin beta4 Antibody detects endogenous levels of total integrin β4 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1812 of human integrin β4 protein. Antibodies are purified peptide affinity chromatography. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2).Integrin β4 pairs with integrin α6 on the cell surface membrane to form the integrin α6β4 heterodimer, an important laminin receptor that is essential for hemidesmosome formation and the support of stable adhesions between basal epithelial cells and the basement membrane (4,5). Integrin β4 is an important component in several growth factor induced signaling pathways that are involved in tumorigenesis and invasive cell growth (6,7). Alternate Names CD104; CD104 antigen; GP150; Integrin beta-4; integrin beta-4 subunit; integrin subunit beta 4; integrin, beta 4; ITB4; ITGB4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 210 SOURCE: Rabbit