CST, 4710S, TRAF1 (1F3) Rat Monoclonal Antibody

Monoclonal Antibody for studying TRAF1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, TRAF1 (1F3) Rat Monoclonal Antibody (CST #4710) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:25 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TRAF1 (1F3) Rat Monoclonal Antibody detects endogenous levels of total TRAF1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a TRAF1-GST fusion protein and purified by protein G affinity chromatography. Background TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. Alternate Names EBI6; Epstein-Bar virus-induced protein 6; Epstein-Barr virus-induced protein 6; MGC:10353; TNF receptor associated factor 1; TNF receptor-associated factor 1; TRAF1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rat IgG2a