CST, 4711S, Integrin alphaV Antibody

Polyclonal Antibody for studying Integrin Alpha V. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin alphaV Antibody (CST #4711) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Integrin alphaV Antibody detects endogenous levels of total integrin αV protein. Species Reactivity: Human, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg108 of human integrin αV. Antibodies are purified by peptide affinity chromatography. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and eight β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Several αV subfamily members, including αVβ3, αVβ5, and αVβ1, are highly expressed in active endothelial cells and cancer cells (3-6), where they play a critical role in angiogenesis and tumor metastasis (7-9). Therefore, interest has focused on αV integrin as a key therapeutic target in cancer treatment (10-12). Alternate Names antigen identified by monoclonal antibody L230; CD51; DKFZp686A08142; Integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; integrin subunit alpha V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITAV; ITGAV; MSK8; Vitronectin receptor; Vitronectin receptor subunit alpha; VNRA; VTNR Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 135, 140 SOURCE: Rabbit