CST, 4719S, Snail (SN9H2) Rat Monoclonal Antibody

Monoclonal Antibody for studying Snail. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Snail (SN9H2) Rat Monoclonal Antibody (CST #4719) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Snail (SN9H2) Rat Monoclonal Antibody detects endogenous levels of total Snail. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant human Snail protein. The epitope has been mapped to residues surrounding Ala125. Background Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (1-3). Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines (4,5). In addition, Snail blocks the cell cycle and confers resistance to cell death (6). Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function (7-10). Alternate Names dJ710H13.1; Protein sna; Protein snail homolog 1; SLUGH2; SNA; SNAH; SNAI1; SNAIL; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family transcriptional repressor 1; snail family zinc finger 1; snail homolog 1; snail homolog 1 (Drosophila); Snail1; Zinc finger protein SNAI1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rat IgG2a