CST, 4725S, p48 Primase (8G10) Rat Monoclonal Antibody

Monoclonal Antibody for studying p48 Primase. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, p48 Primase (8G10) Rat Monoclonal Antibody (CST #4725) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity p48 Primase (8G10) Rat Monoclonal Antibody detects endogenous levels of total p48 primase protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant human p48 primase. Background Initiation of eukaryotic DNA replication is a stringently regulated process that requires the cooperation of many proteins and protein complexes to occur efficiently, at the origins of replication, and once per cell cycle. The initiation of DNA replication requires a protein complex composed of two DNA polymerase α subunits and a pair of primase subunits. Primase activity catalyzes synthesis of an RNA/DNA primer (initiator DNA) on the leading and lagging strands, while polymerase activity extends the initiator DNA (1). The 48 and 58 kDa primase subunits cooperate in the synthesis of small RNA primers. p48 is the catalytically active subunit (2), while p58 couples p48 to the polymerase to allow the transfer of primers to the active site. The p58 subunit may also play a role in regulation of primer length (3,4). Alternate Names DNA primase 1; DNA primase 49 kDa subunit; DNA primase polypeptide 1; DNA primase small subunit; DNA primase subunit 1; DNA primase subunit 48; MGC12308; p49; PRI1; PRIM1; primase (DNA) subunit 1; primase p49 subunit; primase polypeptide 1, 49kDa; primase, DNA, polypeptide 1 (49kDa) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rat IgG1