CST, 4726T, p58 Primase (8D3) Rat Monoclonal Antibody

Monoclonal Antibody for studying p58 Primase. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, p58 Primase (8D3) Rat Monoclonal Antibody (CST #4726) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity p58 Primase (8D3) Rat Monoclonal Antibody detects endogenous levels of total p58 primase protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with full-length human recombinant p58 primase. Background Initiation of eukaryotic DNA replication is a stringently regulated process that requires the cooperation of many proteins and protein complexes to occur efficiently, at the origins of replication, and once per cell cycle. The initiation of DNA replication requires a protein complex composed of two DNA polymerase α subunits and a pair of primase subunits. Primase activity catalyzes synthesis of an RNA/DNA primer (initiator DNA) on the leading and lagging strands, while polymerase activity extends the initiator DNA (1). The 48 and 58 kDa primase subunits cooperate in the synthesis of small RNA primers. p48 is the catalytically active subunit (2), while p58 couples p48 to the polymerase to allow the transfer of primers to the active site. The p58 subunit may also play a role in regulation of primer length (3,4). Alternate Names dJ422B11.1.1; DNA primase 58 kDa subunit; DNA primase large subunit; DNA primase polypeptide 2; DNA primase subunit 2; DNA primase subunit p58; MGC75142; p58; polypeptide 2A, p58; PRI2; PRIM2; PRIM2A; primase (DNA) subunit 2; primase polypeptide 2A, 58kDa; primase, DNA, polypeptide 2 (58kDa); primase, polypeptide 2A (58kD) Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rat IgG2a