CST, 4729S, TRAF3 Antibody

Polyclonal Antibody for studying TRAF3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRAF3 Antibody (CST #4729) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRAF3 Antibody detects endogenous levels of total TRAF3 protein. Cross reactivity was not detected with other family members at physiological conditions. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a central region within TRAF3. Antibodies were purified by protein A and peptide affinity chromatography. Background TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. Alternate Names CAP-1; CAP1; CD40 associated protein 1; CD40 binding protein; CD40 receptor associated factor 1; CD40 receptor-associated factor 1; CD40-binding protein; CD40BP; CRAF1; IIAE5; LAP1; LMP1 associated protein; LMP1-associated protein 1; RING-type E3 ubiquitin transferase TRAF3; RNF118; TNF receptor associated factor 3; TNF receptor-associated factor 3; TRAF3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 62 SOURCE: Rabbit