CST, 47310T, S100A8 (E4F8V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying S100A8 mouse. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, S100A8 (E4F8V) Rabbit Monoclonal Antibody (CST #47310) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:1600 - 1:6400 Immunohistochemistry (Paraffin): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 70802 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity S100A8 (E4F8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total S100A8 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse S100A8 recombinant protein. Background S100A8 and S100A9 are calcium-binding proteins that form a noncovalent heterodimer present in monocytes, neutrophils, macrophages, and some epithelial cells (1,2). S100A8 and S100A9 are secreted by a tubulin-dependent mechanism during inflammatory conditions and have antimicrobial and chemotactic functions (3-5). Extracellular S100A8/S100A9 also induces an inflammatory response in endothelial cells, including induction of proinflammatory chemokines and adhesion molecules and increased vascular permeability (6). S100A8/S100A9 induces and recruits myeloid-derived suppressor cells (MDSC) in tumor-bearing mice (7). MDSC produce additional S100A8/S100A9 themselves, resulting in a positive feedback mechanism that sustains MDSC accumulation (7). S100A8/S100A9 is also highly expressed in psoriatic skin, where it directly upregulates transcription of complement protein C3, which contributes to disease (8). In addition, tumor-infiltrating myeloid cells induce expression of S100A8 and S100A9 in cancer cells, which increases invasiveness and metastasis (9). Alternate Names 60B8A; 60B8Ag; AI323541; B8A; B8Ag; Caga; Calgranulin-A; CFA; CFAg; Chemotactic cytokine CP-10; chemotactic S100 protein; CP-1; CP-10; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; Mrp8; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100a8; S10A8 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 9 Source/Isotype: Rabbit IgG