CST, 4736S, ORC2 (3G6) Rat Monoclonal Antibody

Monoclonal Antibody for studying ORC2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, ORC2 (3G6) Rat Monoclonal Antibody (CST #4736) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity ORC2 (3G6) Rat Monoclonal Antibody recognizes endogenous levels of total ORC2 protein. This antibody does not cross-react with other ORC subunits. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant human ORC2 protein. Background The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7). Alternate Names ORC2; ORC2L; origin of replication 2-like; origin recognition complex protein 2 homolog; Origin recognition complex subunit 2; origin recognition complex, subunit 2 homolog; origin recognition complex, subunit 2-like (yeast) Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rat IgG2a