CST, 47387S, PAPD5 (E9A5D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PAPD5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PAPD5 (E9A5D) Rabbit Monoclonal Antibody (CST #47387) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PAPD5 (E9A5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAPD5 protein. This antibody detects 170 and 120 kDa proteins of unknown identity in some cell lines. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly74 of human PAPD5 protein. Background PAP-associated domain-containing protein 5 (PAPD5) and PAPD7, also known as TENT4B and TENT4A, respectively, are poly(A) polymerases involved in a layer of mRNA regulation by depositing guanosine residues on the poly(A) tail. This process, called mixed tailing, interferes with the deadenylase CCR4-NOT complex from efficiently deadenylating the poly(A) tail (1). PAPD5 and PAPD7 form a complex with ZCCHC14 and play a role in stabilization of hepatitis RNA (2-4). Small molecule inhibitors of PAPD5 and PAPD7 are being pursued for treatment against hepatitis B by inhibiting their polyadenylase activity (5,6). Alternate Names FLJ40270; GLD4; Non-canonical poly(A) RNA polymerase PAPD5; PAP associated domain containing 5; PAP-associated domain-containing protein 5; PAPD5; poly(A) RNA polymerase D5, non-canonical; TENT4B; Terminal guanylyltransferase; terminal nucleotidyltransferase 4A; Terminal nucleotidyltransferase 4B; Terminal uridylyltransferase 3; Topoisomerase-related function protein 4-2; TRF4-2; TUT3; TUTase 3 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG