CST, 47448SF, TNFRSF8/CD30 (E4L4I) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD30. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, TNFRSF8/CD30 (E4L4I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #47448) is ready to ship. Product Usage Information This product is the carrier-free version of product #54535. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 54535 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TNFRSF8/CD30 (E4L4I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TNFRSF8/CD30 protein. This antibody is predicted to detect CD30V, which is a truncated isoform that consists of the CD30 cytoplasmic domain. Non-specific staining was observed in the epithelium of mouse prostate tissue. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu500 of human TNFRSF8/CD30 protein. Background TNFRSF8/CD30 is a type-I transmembrane glycoprotein that is a member of the TNFR superfamily. CD30 is synthesized as a precursor protein that undergoes extensive post-translational modification before becoming embedded in the plasma membrane as a 120-kDa transmembrane protein (1,2). The expression of CD30 is upregulated in activated T cells and may trigger costimulatory signaling pathways upon its engagement (3,4). While its expression is normally restricted to subsets of activated T cells and B cells, CD30 expression is robustly upregulated in hematologic malignancies, such as Hodgkin lymphoma (HL), anaplastic large cell lymphoma (ALCL), and adult T-cell leukemia, thus making it an attractive target for therapeutic intervention (5,6). Research studies have suggested that in certain disease contexts, CD30 recruits TRAF2 and TRAF5 adaptor proteins to drive NF-kappa B activation, aberrant cell growth, and cytokine production (7-9). CD30 signaling is also regulated by TACE-dependent proteolytic cleavage of its ectodomain, which results in reduced CD30L-dependent activation of CD30 cells (10,11). Alternate Names CD30; CD30 antigen; CD30L receptor; cytokine receptor CD30; D1S166E; Ki-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNF receptor superfamily member 8; TNFRSF8; TNR8; Tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 90, 120 Source/Isotype: Rabbit IgG