CST, 4747S, TRA-2-54 (2J) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Alkaline phosphatase. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRA-2-54 (2J) Mouse Monoclonal Antibody (CST #4747) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRA-2-54 (2J) Mouse Monoclonal Antibody recognizes endogenous levels of total TRA-2-54 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with 2102Ep human embryonal carcinoma cells. Background The family of alkaline phosphatases in humans is comprised of four members: intestinal, placental, placental-like (germ cell type), and tissue nonspecific. The tissue nonspecific isozyme is also known as the liver/bone/kidney alkaline phosphatase due to its expression in these tissues. It is also highly expressed in embryonic stem (ES) and embryonic carcinoma (EC) cells and is lost as these cells undergo differentiation (1,2). The TRA-2-54 (2J) Mouse mAb specifically detects this isozyme and does not detect the other family members, making it a useful tool for tracking the pluripotency status of ES and EC cells in culture. Alternate Names Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, biomineralization associated; alkaline phosphatase, liver/bone/kidney; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; AP-TNAP; APTNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; PPBT; tissue non-specific alkaline phosphatase; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; TNALP; TNAP; TNSALP Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Mouse IgG1