CST, 47507T, FGL1 (E8V9Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FGL1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, FGL1 (E8V9Q) Rabbit Monoclonal Antibody (CST #47507) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 84617 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity FGL1 (E8V9Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total FGL1 protein. This antibody detects a faint cross-reactive band around 27 kDa of unknown identity in some cell extracts. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human FGL1 protein. Background FGL1 (fibrinogen-like protein 1, also known as hepassocin) is a secreted protein mainly expressed in liver and its expression is further enhanced following acute liver injury (1-3). FGL1 mice have multiple metabolic abnormalities and are more prone to develop hepatocellular carcinoma under certain experimental conditions, suggesting FGL1 has diverse functions (4,5). More recently, FGL1 was found to be highly expressed in human cancer cells and function as an inhibitory ligand for LAG3. Blockade of the FGL1-LAG3 interaction stimulates tumor immunity in mouse models. In addition, a high plasma FGL1 level in human cancer patients is associated with a poor prognosis and resistance to anti-PD-1/PD-L1 immunotherapy (6). Alternate Names FGL1; fibrinogen like 1; fibrinogen-like 1; Fibrinogen-like protein 1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1; HP-041; HPS; LFIRE-1; LFIRE1; Liver fibrinogen-related protein 1; MGC12455 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG