CST, 4755S, SSEA4 (MC813) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, SSEA4 (MC813) Mouse Monoclonal Antibody (CST #4755) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Flow Cytometry (Live): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity SSEA4 (MC813) Mouse Monoclonal Antibody detects endogenous levels of SSEA4 antigen. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by injecting animals with human embryonal carcinoma 2102Ep cl.2A6 cells. Background SSEA4 (stage-specific embryonic antigen 4) is a glycolipid carbohydrate epitope expressed on the surface of human teratocarcinoma stem cells, human embryonic germ cells, and human embryonic stem cells (1). Expression of human SSEA4 decreases following differentiation of human embryonal carcinoma cells. Expression of the SSEA4 antigen is absent in murine pluripotent cells, but increases following differentiation (1,2). Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): N/A Source/Isotype: Mouse IgG3