CST, 47577S, MLLT3 (E5Z7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MLLT3. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MLLT3 (E5Z7U) Rabbit Monoclonal Antibody (CST #47577) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MLLT3 (E5Z7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MLLT3 protein. This antibody detects 19 and 100 kDa proteins of unknown origin. This antibody nonspecifically labels cytoplasmic puncta. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala217 of human MLLT3 protein. Background The super elongation complex (SEC) plays a critical role in regulating RNA polymerase II (RNAPII) transcription elongation (1). The SEC is composed of AFF4, AFF1/AF4, MLLT3/AF9, and MLLT1/ENL proteins. The pathogenesis of mixed lineage leukemia is often associated with translocations of the SEC subunits joined to the histone H3 Lys4 methyltransferase mixed lineage leukemia ( ) gene (1-4). The SEC has been found to contain RNAPII elongation factors eleven-nineteen lysine-rich leukemia (ELL), ELL2, and ELL3, along with the associated factors EAF1 and EAF2, which can increase the catalytic rate of RNAPII transcription , (1,2,5-7). The SEC positive transcription elongation factor b (P-TEFb) phosphorylates the carboxy-terminal domain within the largest subunit of RNAP II at Ser2 of the heptapeptide repeat. The SEC negative transcription elongation factors, DRB-induced stimulating factor (DSIF) and negative elongation factor (NELF), signal the transition from transcription initiation and pausing to productive transcription elongation (2,8-10). The chromosomal translocation of with the members of the SEC leads to SEC recruitment to MLL regulated genes, such as the highly developmentally regulated genes, implicating the misregulation and overexpression of these genes as underlying contributors to leukemogenesis (1,2,9,11). Alternate Names AF9; ALL1-fused gene from chromosome 9 protein; FLJ2035; KMT2A/MLLT3 fusion protein; MLLT3; MLLT3 super elongation complex subunit; myeloid/lymphoid or mixed-lineage leukemia; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; Protein AF-9; translocated to 3; translocated to, 3; YEATS domain-containing protein 3; YEATS3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG