CST, 47600S, SLIT2 (E8W7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLIT2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SLIT2 (E8W7Y) Rabbit Monoclonal Antibody (CST #47600) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SLIT2 (E8W7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLIT2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1463 of human SLIT2 protein. Background SLIT2 is an extracellular matrix-associated SLIT family member that functions as a ligand for roundabout (ROBO) family receptors (1). Activation of ROBO receptors by SLIT2 regulates various biological processes, including promoting cellular senescence via WNT inhibition (2), suppressing migration by enhanced β-catenin/E-cadherin association, regulating actin polymerization (4, 5), and suppressing cell proliferation induced by SDF1 and MCP1 (6, 7). In development, the SLIT-ROBO pathways play important roles in neuronal axon guidance, angiogenesis, and both kidney and mammary gland organogenesis. SLIT2 expression has been reported to suppress cancer cell growth, invasion, and metastasis, suggesting that modulation of SLIT2-ROBO signaling may have therapeutic potential in cancer biology (8, 9). Alternate Names FLJ14420; SLIL3; slit guidance ligand 2; slit homolog 2 (Drosophila); Slit homolog 2 protein; Slit homolog 2 protein C-product; Slit homolog 2 protein N-product; Slit-2; SLIT2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG