CST, 47603SF, POU2F2 (F3B7M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying POU2F2. Validated for WB,IF,IF,F. Highly specific and rigorously validated in-house, POU2F2 (F3B7M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #47603) is ready to ship. Product Usage Information This product is the carrier free version of product #49509. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 49509 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity POU2F2 (F3B7M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total POU2F2 protein. This antibody is predicted to detect multiple human and mouse POU2F2 isoforms. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human POU2F2 protein. Background POU domain, class 2, transcription factor 2 (POU2F2), also known as Oct-2, is a transcription factor in the POU protein family (1-3). POU proteins contain two subunits: an N-terminal POU-specific domain and a C-terminal homeobox domain (1-3). Due to alternate splicing, POU2F2 has five human isoforms and seven mouse isoforms (3). POU2F2 is normally expressed in B cells and B cell lineage tumor cells, and is involved in B cell proliferation and differentiation by binding to immunoglobulin gene promoters (2-4). POU2F2 has also been shown to have a tumor-promoting role in some cancers, as well as potentially promoting oncogene-induced senescence (OIS) escape in early cancer progression (5,6). Alternate Names homeobox protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; OCT2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2; PO2F2; POU class 2 homeobox 2; POU domain, class 2, transcription factor 2; POU2F2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG