CST, 4762S, Filamin A Antibody

Polyclonal Antibody for studying Filamin A. Validated for Western Blotting. Highly specific and rigorously validated in-house, Filamin A Antibody (CST #4762) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Filamin A Antibody detects endogenous levels of total filamin A protein. This antibody may cross-react with filamin B and C. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to human filamin A. Antibodies are purified by protein A and peptide affinity chromatography. Background Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (α-actinin domain) located at the amino terminus is followed by as many as 24 tandem repeats of about 96 residues and the dimerization domain is located at the carboxy terminus. In addition to actin filaments, filamins associate with other structural and signaling molecules such as β-integrins, Rho/Rac/Cdc42, PKC and the insulin receptor, primarily through the carboxy-terminal dimerization domain (1-3). Filamin A, the most abundant, and filamin B are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Filamin A is phosphorylated by PAK1 at Ser2152, which is required for PAK1-mediated actin cytoskeleton reorganization (4). Alternate Names ABP-280; ABPX; actin binding protein 280; Actin-binding protein 280; Alpha-filamin; CSBS; CVD1; DKFZp434P031; Endothelial actin-binding protein; epididymis secretory sperm binding protein; FGS2; filamin 1; filamin A; filamin A, alpha; Filamin-1; Filamin-A; FLN; FLN-A; FLN1; FLNA; FMD; MNS; NHBP; Non-muscle filamin; OPD; OPD1; OPD2; XLVD; XMVD Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 280 SOURCE: Rabbit