CST, 47729T, RBM10 (E5V5K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBM10. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, RBM10 (E5V5K) Rabbit Monoclonal Antibody (CST #47729) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RBM10 (E5V5K) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBM10 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His919 of human RBM10 protein. Background RBM10 is an RNA binding protein and a paralog to tumor suppressor RBM5 (1,2). Alternative splicing creates two highly expressed variants that differ by one exon. RBM10 controls alternative splicing and exon skipping of mRNAs, including Fas and Bcl-x (3,4). RBM10 has been shown to affect apoptosis via up regulation of TNF-α mRNA (5). In lung cancer, RBM10 mutations disrupt the splicing of NUMB, a regulator of Notch signaling (6,7). Fusion of the gene with TFE3 has been found in renal cell carcinoma (8-9). Mutations of RBM10 causes TARP syndrome, an x-linked, lethal disorder characterized by various developmental defects (10-12). Alternate Names DXS8237E; epididymis secretory sperm binding protein; G patch domain-containing protein 9; GPATC9; GPATCH9; KIAA0122; MGC1132; MGC997; RBM10; RNA binding motif protein 10; RNA-binding motif protein 10; RNA-binding protein 10; RNA-binding protein S1-1; S1-1; TARPS; ZRANB5 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 110, 135 Source/Isotype: Rabbit IgG