CST, 4775T, Tid-1 (RS13) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Tid-1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Tid-1 (RS13) Mouse Monoclonal Antibody (CST #4775) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Tid-1 (RS13) Mouse Monoclonal Antibody detects endogenous levels of the short and long variants of Tid-1. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human Tid-1 protein. Antibody is supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 mg/ml BSA and 50% glycerol. Background Human Tid-1 is a human orthologue of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l (2) tid and is a member of the DnaJ family of proteins that serve as co-chaperones to Hsp70 proteins (1). These proteins are characterized by a J domain, a highly conserved tetrahelical domain that binds to Hsp70 chaperones and activates their ATPase activity. Hsp70 and their associated chaperones mediate a variety of activities including the folding of newly synthesized polypeptides, the translocation of proteins across membranes and assembly of multimeric protein complexes. Two alternatively spliced variants exist for human Tid-1 ,designated hTID-1s and hTID-1L, both which contain the J domain, localize to the mitochondrial matrix, and co-immunoprecipitate with Hsp70. Expression of Tid-1L increases apoptosis induced by the DNA damaging agent mitomycin c (MMC) and by TNF-alpha, and that activity is dependent on its J domain. In contrast, expression of Tid-1S reduces apoptosis by these agents. Tid-1 orthologues are also found in mouse (mTid-1) and rat (rTid-1) (2,3). The mouse orthologue was originally identified though its interaction with p120 GTPase-activating protein (GAP), raising the possiblity that Tid-1 helps regulates the confirmation, activity, or subcellular localization of GAP (3). Alternate Names DnaJ (Hsp40) homolog, subfamily A, member 3; DnaJ heat shock protein family (Hsp40) member A3; DnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; DNAJA3; DNJA3; FLJ45758; HCA57; Hepatocellular carcinoma-associated antigen 57; hTid-1; TID1; tumorous imaginal discs homolog; Tumorous imaginal discs protein Tid56 homolog Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 37 Tid-1s. 40 Tid-1L. Source/Isotype: Mouse IgG1