CST, 47778T, LAMP3/CD208 (E6E5U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAMP3. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP3/CD208 (E6E5U) Rabbit Monoclonal Antibody (CST #47778) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 88687 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity LAMP3/CD208 (E6E5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMP3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro257 of human LAMP3/CD208 protein. Background Lysosome-associated membrane proteins (LAMPs) are a family of proteins present predominantly on the membrane of lysosomes. Five members have been identified so far (LAMP1, LAMP2, LAMP3, CD68/Macrosialin/LAMP4, and BAD-LAMP/LAMP5). LAMP proteins are involved in many different aspects of cell biology and can influence cellular processes, such as phagocytosis, autophagy, lipid transport, and aging. Their expression varies among different tissues (1). LAMP3, also known as CD-LAMP and CD208, is highly expressed only in certain cell types and differentiation stages. LAMP3 is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation, but is not expressed by DCs in mouse (2-4). Its expression is associated with poor prognosis of certain tumors, and the locus where it is encoded was identified as a risk factor for Parkinson's disease (PD) (5). Alternate Names CD208; DC LAMP; DC-LAMP; DC-lysosome-associated membrane glycoprotein; DCLAMP; LAMP; LAMP-3; LAMP3; lysosomal associated membrane protein 3; Lysosomal-associated membrane protein 3; Lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70, 140 Source/Isotype: Rabbit IgG