CST, 4783S, hnRNP LL Antibody

Polyclonal Antibody for studying hnRNPLL. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, hnRNP LL Antibody (CST #4783) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity hnRNP LL Antibody detects endogenous levels of total hnRNP LL protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human hnRNP LL protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL) is a nuclear RNA-binding protein that shares 69% amino acid homology with hnRNP L, a component of the hnRNP complex that regulates mRNA formation, packaging, and processing (1). hnRNP LL is induced in activated T cells and functions as a critical regulator of alternative splicing of CD45, a tyrosine phosphatase crucial for T cell development and activation (2). hnRNP LL also regulates the splicing of CD44 and Stat5a (2). The four isoforms of hnRNP LL are generated by alternative splicing and are widely expressed in human tissues (3). Alternate Names heterogeneous nuclear ribonucleoprotein L like; Heterogeneous nuclear ribonucleoprotein L-like; HNRLL; hnRNPLL; HNRPLL; SRRF; stromal RNA regulating factor; Stromal RNA-regulating factor Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 61 SOURCE: Rabbit