CST, 47857S, DDX39A (F7T4J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DDX39A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DDX39A (F7T4J) Rabbit Monoclonal Antibody (CST #47857) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DDX39A (F7T4J) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX39A protein. This antibody does not cross-react with UAP56 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro22 of human DDX39A protein. Background The gene is found in the central MHC region and encodes a member of the DEAD-box family of RNA helicases (1). Also known as DDX39B and BAT1, UAP56 functions as an ATP-dependent splicing factor and RNA helicase in the evolutionary conserved transcription/export (TREX) complex. The TREX complex is recruited to sites of active transcription, where it travels along the length of the gene with RNA polymerase II and exports resulting mRNAs to the cytoplasm (2-8). DDX39, also known as DDX39A, is a DEAD-box helicase highly homologous to UAP56 and is upregulated in lung squamous cell cancers (9,10). DDX39 has been shown to bind to TRF2 to regulate telomere protection (11). Furthermore, DDX39 is overexpressed in several cancer types, which is associated with poor prognosis (12-15). Both UAP56 and DDX39 are hijacked by various viral replication machineries to enable viral reproduction and mRNA export (16-18). UAP56 and DDX39 have also been implicated in promoting the AR-V7 splice variant in advanced prostate cancers (19). Alternate Names ATP-dependent RNA helicase DDX39; ATP-dependent RNA helicase DDX39A; BAT1; BAT1L; DDX39; DDX39A; DDXL; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 transcript; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A; DEAD box protein 39; DEAD-box helicase 39A; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39; DExD-box helicase 39A; DX39A; MGC18203; MGC8417; Nuclear RNA helicase URH49; nuclear RNA helicase, DECD variant of DEAD box family; UAP56-related helicase, 49 kDa; URH49 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG