CST, 47935S, PYCR1 Antibody

Polyclonal Antibody for studying PYCR1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, PYCR1 Antibody (CST #47935) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PYCR1 Antibody recognizes endogenous levels of total PYCR1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PYCR1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Mitochondrial pyrroline-5-carboxylate reductase 1 (PYCR1) catalyzes the last step in proline biosynthesis (1). Research studies show that proline is a limiting amino acid to clear cell renal cell carcinoma (ccRCC). expression is induced in kidney cancer cells to compensate for the proline shortage and maintain cancer cell growth. Furthermore, knockdown inhibits ccRCC cell proliferation in the absence of proline, suggesting this enzyme as a potential cancer therapeutic target (2). In addition, breast cancer cells also express high levels of PYCR1 (2,3). Proline is restrictive to invasive ductal breast carcinoma cells, indicating proline vulnerability in breast cancer formation (2). Alternate Names ARCL2B; ARCL3B; P5C; P5C reductase 1; P5CR; P5CR 1; P5CR1; PIG45; PP222; PRO3; proliferation-inducing protein 45; PYCR; PYCR1; pyrroline-5-carboxylate reductase 1; Pyrroline-5-carboxylate reductase 1, mitochondrial Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30, 32 SOURCE: Rabbit