CST, 47961T, MTX1 (E5L8N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MTX1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MTX1 (E5L8N) Rabbit Monoclonal Antibody (CST #47961) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MTX1 (E5L8N) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTX1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro400 of human MTX1 protein. Background Metaxin-1 (MTX1) and Metaxin-2 (MTX2) are part of the sorting and assembly machinery (SAM) that has been associated with import of β-barrel proteins through the mitochondrial outer membrane (1-3). MTX1 and MTX2 can also function in mitochondrial trafficking in neurons (4). Studies of cells with a loss of MTX1 have shown that MTX1 is required for some forms of cell death, including TNF-α-induced cell death (5). Subsequent studies found that MTX1 and MTX2 can regulate TNF-α mediated apoptosis through association with the apoptotic effector protein Bak, and that phosphorylation of MTX1 controls Bak activation (6,7). MTX1 has also been shown to be targeted for basal piecemeal mitophagy in an LC3C- and p62/SQSMT1-dependent manner (8). MTX1 was identified as a regulator of sorafenib resistance in hepatocellular carcinoma by augmenting autophagy (9). Alternate Names metaxin 1; Metaxin-1; Mitochondrial outer membrane import complex protein 1; MTX; MTX1; MTXN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG