CST, 48103SF, Lyn (C13F9) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Lyn. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Lyn (C13F9) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #48103) is ready to ship. Product Usage Information This product is the carrier free version of product #2796. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2796 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Lyn (C13F9) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Lyn protein. This antibody does not cross-react with any other Src-family members. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human Lyn. Background Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8). Alternate Names FLJ26625; JTK8; Lck/Yes-related novel protein tyrosine kinase; LYN; LYN proto-oncogene, Src family tyrosine kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG