CST, 48110S, Cytochrome c (6H2.B4) Mouse Monoclonal Antibody (InTraSeq™ 3' Conjugate 3089)

Monoclonal Antibody for studying cytochrome C. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Cytochrome c (6H2.B4) Mouse Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3089) (CST #48110) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Cytochrome c (6H2.B4) Mouse mAb (InTraSeq™ 3' Conjugate 3089) Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant rat cytochrome c protein. Background Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3). Alternate Names CYC; CYCS; Cytochrome c; cytochrome c, somatic; HCS; THC4 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1