CST, 48111S, Cadherin-6 (D3T3I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cadherin-6. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Cadherin-6 (D3T3I) Rabbit Monoclonal Antibody (CST #48111) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 84079 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cadherin-6 (D3T3I) Rabbit Monoclonal Antibody recognizes endogenous levels of total cadherin-6 protein. Staining of peripheral nerves and limited staining of immune cells has been observed. The specificity of this staining is unknown. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp761 of human cadherin-6 protein. Background Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). Cadherin-6, also known as kidney cadherin (K-Cadherin, CDH6) is a type II classical cadherin. While it was reported to have a tumor suppressor function in cholangiocarcinoma (9), cadherin-6 expression was shown to be a marker of epithelial mesenchymal transition, and positively correlated with stage and metastasis of papillary thyroid carcinoma (10, 11). In related studies, cadherin-6 was shown to interact with GABARAP and related proteins to restrain autophagy, thereby promoting metastatic behavior (12). Cadherin-6 has since been proposed as an antibody-drug conjugate target for the treatment of ovarian and renal cancers (13). Alternate Names CAD6; CADH6; cadherin 6; cadherin 6, K-cadherin (fetal kidney); cadherin 6, type 2, K-cadherin (fetal kidney); Cadherin-6; cadherin, fetal kidney; CDH6; K-cadherin; KCAD; Kidney cadherin Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG