CST, 48122T, OCA-T2 (F5A7Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OCA-T2 (COLCA2 / C11orf93). Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, OCA-T2 (F5A7Z) Rabbit Monoclonal Antibody (CST #48122) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity OCA-T2 (F5A7Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total OCA-T2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human OCA-T2 protein. Background OCA-T2 (COLCA2, POU2AF3) and its paralog OCA-T1 (C11orf53) were first identified by single-cell RNA sequencing (scRNAseq) to be co-expressed with the transcription factor POU2F3, in specialized epithelial cells called tuft cells (1). Tuft cells exist as solitary chemosensory cells within mucosal epithelium and serve to coordinate interactions between immune and neuronal cells (2). Systematic gene knockdown experiments demonstrated chromatin-level interactions between OCA-T1, OCA-T2, and POU2F3 that appeared essential for tuft cell lineage determination (1). Notably, the oncogenic transformation of tuft cells has been identified as a defining feature of a distinct small cell lung cancer (SCLC) subtype known as SCLC-P, defined by their dependence on POU2F3 expression (3). studies showed that targeted knockdown of OCA-T1Â and OCA-T2 suppressed growth of cultured SCLC-P cell lines, supporting a role for OCA-T1 and OCA-T2 in SCLC (4). Subsequently, it has been observed that gene fusions between or and are defining features of recently discovered aggressive sarcoma subtypes (5-7). Alternate Names C11orf93; cancer susceptibility candidate 13; Cancer susceptibility candidate protein 13; CASC13; COLC2; COLCA2; colorectal cancer associated 2; colorectal cancer associated two; Colorectal cancer-associated protein 2; LOH11CR1G; OCAT2; POU class 2 homeobox associating factor 3; POU2AF3; Protein OCA-T2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG