CST, 48139S, Cleaved Caspase-4 (Asp270)/Caspase-5 (Asp327) (F3Q2F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CASP4 (Asp270,C-term) cleaved/CASP5 (Asp327) cleaved. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-4 (Asp270)/Caspase-5 (Asp327) (F3Q2F) Rabbit Monoclonal Antibody (CST #48139) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved Caspase-4 (Asp270)/Caspase-5 (Asp327) (F3Q2F) Rabbit Monoclonal Antibody recognizes transfected levels of caspase-4 protein only when cleaved between Asp270 and Ser271 and transfected levels of caspase-5 protein only when cleaved between Asp327 and Ser328. This antibody is predicted to detect additional cleavage products of caspase-4 and caspase-5 produced following cleavage of their prodomain sites. This antibody detects a 48 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp270 of human caspase-4 protein. Background Canonical activation of the inflammatory necrotic form of cell death, pyroptosis, occurs following inflammasome-mediated activation of caspase-1, leading to cleavage of gasdermin D (1,2). Alternatively, non-canonical cleavage of gasdermin D can be triggered by direct binding of bacterial lipopolysaccharide (LPS) to additional caspases, specifically mouse caspase-11 or human caspase-4 and caspase-5 (2-4). Unlike caspase-4, caspase-5 expression is strongly regulated by inflammatory signals such as LPS and interferon-γ (5). Caspase-4 and caspase-5 activation requires proteolytic processing between three domains: a cascade activation and recruitment domain (CARD), followed by the large and small subunits that form the active protease (6-10). LPS binding to the CARD domain results in cleavage of caspase-4 between the large and small subunits located at Asp270 and Asp289 and at Asp59 in the prodomain (11,12). Equivalent sites are found on caspase-5 at Asp327, Asp346, and Asp137 (13). Alternate Names apoptotic cysteine protease Mih1/TX; CASP-4; CASP-5; CASP4; CASP5; caspase 4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; caspase 5; caspase 5, apoptosis-related cysteine peptidase; caspase 5, apoptosis-related cysteine protease; Caspase-4; Caspase-4 subunit 1; Caspase-4 subunit 2; Caspase-5; Caspase-5 subunit p10; Caspase-5 subunit p20; Caspase4; ICE and Ced-3 homolog 2; ICE(rel)-II; ICE(rel)-III; ICE(rel)II; ICE(rel)III; ICEREL-II; ICEREL-III; ICH-2; ICH-3; ICH2; ICH3; MGC141966; Mih1; Mih1/TX; Protease ICH-2; Protease ICH-3; Protease TX; Protease TY; TX; TY protease Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 36 (casp5), 30 (casp4) Source/Isotype: Rabbit IgG