CST, 4813S, USP18 (D4E7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying USP18. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, USP18 (D4E7) Rabbit Monoclonal Antibody (CST #4813) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity USP18 (D4E7) Rabbit Monoclonal Antibody detects endogenous levels of total USP18 protein. The doublet band detected by western blot represents full length (39 kDa) and amino-terminal deleted derivative of USP18 (8). Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro45 of human USP18 protein. Background Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes (1,2). USP18 (also known as UBP43) is a deubiquitinase best known for catalyzing the removal of ISG15, an interferon-regulated ubiquitin-like protein, from conjugated proteins (3). Removal of ISG15 from target proteins by the USP18 peptidase maintains the critical cellular balance of ISG15-conjugated proteins important for normal development and brain function (4,5). Following induction by IFN or LPS (6), USP18 binds the INF receptor subunit IFNAR2 and inhibits signal transduction through the Jak/Stat pathway (7). USP18 regulation of IFN signaling inhibits IFN-mediated apoptosis and does not necessarily rely on USP18 peptidase activity (8). As the therapeutic use of recombinant IFN can lead to refractory IFN signaling and a less effective response, the combination of IFN treatment and regulation of USP18 expression may produce a more positive outcome (9). Alternate Names 43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; ISG43; PTORCH2; ubiquitin specific peptidase 18; ubiquitin specific protease 18; Ubl carboxyl-terminal hydrolase 18; Ubl thioesterase 18; ubl thiolesterase 18; UBP18; UBP43; USP18 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 34, 39 Source/Isotype: Rabbit IgG