CST, 48159S, FBP2/FBPase 2 (E9M5T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FBPase 2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FBP2/FBPase 2 (E9M5T) Rabbit Monoclonal Antibody (CST #48159) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FBP2/FBPase 2 (E9M5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total FBP2/FBPase 2 protein. This antibody does not cross-react with FBP1/FBPase 1 protein. This antibody detects an 80 kDa nonspecific band. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FBP2/FBPase 2 protein. Background Fructose-1,6-bisphosphatase 2 (FBP2 or FBPase 2) is a rate limiting enzyme in gluconeogenesis. It catalyzes the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate (1,2). FBP2/FBPase 2 expression is inhibited in various soft tissue sarcoma subtypes. Ectopic expression of FBP2/FBPase 2 represses soft tissue sarcoma cell proliferation and tumor growth. Studies show that cytosolic FBP2/FBPase 2 inhibits glycolysis through its catalytic activity, suppressing sarcoma cell proliferation. In addition, nuclear FBP2/FBPase 2 inhibits mitochondrial biogenesis and oxidative phosphorylation independent of its catalytic activity, also contributing to the suppression of sarcoma cell proliferation (2). Alternate Names D-fructose-1,6-bisphosphate 1-phosphohydrolase 2; F16P2; FBP2; FBPase 2; fructose-1,6-bisphosphatase 2; Fructose-1,6-bisphosphatase isozyme 2; fructose-bisphosphatase 2; hexosediphosphatase; MGC142192; muscle FBPase; muscle fructose-bisphosphatase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG