CST, 4817S, STEP Antibody

Polyclonal Antibody for studying STEP. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, STEP Antibody (CST #4817) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity STEP Antibody detects endogenous levels of total STEP61 and may detect STEP46 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human STEP. Antibodies are purified by protein A and peptide affinity chromatography. Background Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer's disease (1). Alternate Names FLJ14427; Neural-specific protein-tyrosine phosphatase; protein tyrosine phosphatase non-receptor type 5; protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched); protein-tyrosine phosphatase striatum-enriched; PTN5; PTPN5; PTPSTEP; STEP; STEP61; striatal-enriched protein tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase; Tyrosine-protein phosphatase non-receptor type 5 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 61 SOURCE: Rabbit