CST, 4819S, GABA(B)R2 (C44A4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GABA(B)R2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GABA(B)R2 (C44A4) Rabbit Monoclonal Antibody (CST #4819) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GABA(B)R2 (C44A4) Rabbit Monoclonal Antibody detects endogenous levels of total GABA(B)R2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gln430 of human GABA(B)R2. Background GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1). Alternate Names EIEE59; FLJ36928; G protein-coupled receptor 51; G-protein coupled receptor 51; GABA-B receptor 2; GABA-B receptor, R2 subunit; GABA-B-R2; GABA-BR2; GABABR2; GABBR2; GABR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid B receptor 2; Gamma-aminobutyric acid type B receptor subunit 2; Gb2; GPR51; GPRC3B; HG20; HRIHFB2099; NDPLHS Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 105 Source/Isotype: Rabbit IgG