CST, 48234T, HSP47/SERPINH1 (E4L3I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SERPINH1. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HSP47/SERPINH1 (E4L3I) Rabbit Monoclonal Antibody (CST #48234) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HSP47/SERPINH1 (E4L3I) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSP47/SERPINH1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HSP47/SERPINH1 protein. Background Serpin peptidase inhibitor, clade H, member 1 (serpin H1), also known as heat shock protein 47 (HSP47), is a collagen specific molecular chaperone localized to the endoplasmic reticulum, essential for collagen biosynthesis. Despite its lack of serine proteinase activity, serpin H1 is a member of the serpin superfamily (1,2). Genetic deletion of or inhibition of its interaction with procollagen has been shown to greatly reduce procollagen secretion from fibroblasts, implicating serpin H1 as a potential target for therapeutic management of fibrosis (3). Moreover, altered serpin H1 expression has been seen in multiple cancer types, including colon carcinoma, head and neck squamous cell carcinoma, and liver hepatocellular carcinoma. Thus, serpin H1 may function as a potential prognostic biomarker for certain cancers (2). Alternate Names 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; CBP1; CBP2; Cell proliferation-inducing gene 14 protein; collagen binding protein 1; Collagen-binding protein; Colligin; colligin-1; colligin-2; gp46; HSP47; OI10; PIG14; PPROM; proliferation-inducing gene 14; RA-A47; rheumatoid arthritis antigen A-47; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); SERPH; serpin family H member 1; Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); SERPINH1; SERPINH2 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG